Growth substrate limitation enhances anaerobic arsenic methylation by <i>Paraclostridium bifermentans</i> strain EML.

ABSTRACT: Microbial arsenic methylation is established as a detoxification process under aerobic conditions (converting arsenite to monomethylated arsenate) but is proposed to be a microbial warfare strategy under anoxic conditions due to the toxicity of its main product, monomethylarsonous acid (MMAs(III)). Here we leveraged a paddy soil-derived anaerobic arsenic methylator, Paraclostridium bifermentans strain EML, to gain insights into this process. Strain EML was inoculated into a series of media involving systematic dilutions of Reinforced Clostridial Broth (RCB) with 25 µM arsenite to assess the impact of growth substrate concentration on arsenic methylation. Growth curves evidenced the sensitivity of strain EML to arsenite, and arsenic speciation analysis revealed the production of MMAs

SUBMITTER: Qiao J 

PROVIDER: S-EPMC11653729 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature