A novel role for protein disulfide isomerase ERp18 in venous thrombosis.

ABSTRACT:

Background

Previous studies using genetically modified mouse models and inhibitors have shown that protein disulfide isomerase (PDI) family plays a significant role in arterial thrombosis. However, their role in venous thrombosis remains unknown. In this study, using gene-modified mouse models, we determined whether PDI family members contribute to venous thrombosis.

Methods

Mice deficient of the PDI family members, including PDI, PDIp, ERp57, PDIr, ERp5, ERp27, ERp29, TMX4, ERdj5, and ERp18, were generated. The venous thrombosis phenotype of these deficient strains was evaluated using an inferior vena cava (IVC) stenosis model. Moreover, the recombinant human ERp18 (rhERp18) protein was generated and its reductase activity was assessed using a Di-E-GSSG method. The effect o

SUBMITTER: He C 

PROVIDER: S-EPMC11653900 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature