Revealing novel CD8⁺ T-cell epitopes from the H5N1 avian influenza virus in HBW/B1 haplotype ducks.

ABSTRACT: The duck CD8⁺ T-cell response effectively defends against H5N1 highly pathogenic avian influenza virus (HPAIV) infection, but the recognized peptide is rarely identified. Here, we found that the ratio of CD8⁺ T cells and the expression of IFN-γ and cytotoxicity-associated genes, including granzyme A/K, perforin and IL2, at 7 days post-infection in peripheral blood mononuclear cells (PBMCs) from B1 haplotype ducks significantly increased in the context of defending against H5N1 AIV infection in vivo. Moreover, similar results were observed in cultured and sorted H5N1 AIV-stimulated duck CD8⁺ T cells in vitro. Next, we selected 109 epitopes as candidate epitopes on the basis of the MHC-I restriction binding peptide prediction website database and further iden

SUBMITTER: Jiao W 

PROVIDER: S-EPMC11653964 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature