Revealing novel and conservative CD8⁺T-cell epitopes with MHC B2 restriction on ALV-J.

ABSTRACT: MHC B2 haplotype chickens have been reported to induce strong immune response against various avian pathogens. However, little is known about the CD8⁺T-cell epitope with MHC B2-restricted on subgroup J avian leukosis virus (ALV-J). In this study, we explored the ALV-J-induced cellular immune response in B2 haplotype chickens in vivo. We found that ALV-J infection significantly increased the proportion of CD8⁺T cells in chickens and up-regulated the expression of cytotoxic genes like Granzyme A and antiviral genes like IFIT5 at 14 days post-infection (dpi). We selected 32 candidate peptides based on the peptide-binding motif and further identified three MHC B2-restricted CD8⁺T epitopes on ALV-J, including Pol_652-660, Gag_374-382, and Gag

SUBMITTER: Li X 

PROVIDER: S-EPMC11654158 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature