Role of TLR4 activation and signaling in bone remodeling, and afferent sprouting in serum transfer arthritis.

ABSTRACT:

Background

In the murine K/BxN serum transfer rheumatoid arthritis (RA) model, tactile allodynia persists after resolution of inflammation in male and partially in female wild type (WT) mice, which is absent in Toll-like receptor (TLR)4 deficient animals. We assessed the role of TLR4 on allodynia, bone remodeling and afferent sprouting in this model of arthritis.

Methods

K/BxN sera were injected into male and female mice with conditional or stable TLR4 deletion and controls. Paw swelling was scored and allodynia assessed by von Frey filaments. At day 28, synovial neural fibers were visualized with confocal microscopy and bone density assayed with microCT. Microglial activity and TLR4 dimerization in spinal cords were examined by immunofluorescence and flow cytometry.

Resu

SUBMITTER: Dos Santos GG 

PROVIDER: S-EPMC11654167 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature