A proteome-wide yeast degron collection for the dynamic study of protein function.

ABSTRACT: Genome-wide collections of yeast strains, known as libraries, revolutionized the way systematic studies are carried out. Specifically, libraries that involve a cellular perturbation, such as the deletion collection, have facilitated key biological discoveries. However, short-term rewiring and long-term accumulation of suppressor mutations often obscure the functional consequences of such perturbations. We present the AID library which supplies "on demand" protein depletion to overcome these limitations. Here, each protein is tagged with a green fluorescent protein (GFP) and an auxin-inducible degron (AID), enabling rapid protein depletion that can be quantified systematically using the GFP element. We characterized the degradation response of all strains and demonstrated its utility by rev

SUBMITTER: Valenti R 

PROVIDER: S-EPMC11654244 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature