MiR-3191 promotes the proliferation and metastasis of hepatocellular carcinoma via regulating PAK6.

ABSTRACT:

Background/aims

microRNAs (miRNAs) contribute to tumorigenesis, progression and drug resistance of hepatocellular carcinoma (HCC). miR-3191 is a newly discovered miRNA, and its function and mechanism of action in biological processes and diseases are not completely understood.

Methods

miR-3191 expression is determined via quantitative real-time polymerase chain reaction. Knockdown and overexpression of miR-3191 influence the proliferation and metastasis of HCC cells, which is measured by Cell Counting Kit-8 assay, Colony Formation assay and Cell metastasis assay. Protein expression is estimated by Western blot. The interplay between miR-3191 and target is validated by dual-luciferase reporter assay.

Results

Here, we show that miR-3191 is upregulated in HCC tissues and

SUBMITTER: Xie A 

PROVIDER: S-EPMC11654304 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature