Integrated network pharmacology and transcriptomics to explore the mechanism of compound Dihuang granule (CDG) protects dopaminergic neurons by regulating the Nrf2/HMOX1 pathway in the 6-OHDA/MPP⁺-induced model of Parkinson's disease.

ABSTRACT:

Background

Parkinson's disease (PD) is a degenerative neurological disease that worsens over time. Ferroptosis has been proven to contribute to PD pathogenesis. CDG exhibits neuroprotective effects. However, CDG's potential mechanism in PD therapy remains uncertain.

Purpose

The purpose of this investigation is to ascertain the specific molecular mechanisms of CDG against neuronal ferroptosis and present an alternative option for PD management.

Methods

Network pharmacology along with LC-MS were used to identify possible targets and candidate pathways. Then RNA-sequencing combined in the in vitro and in vivo experiments were utilized to validate these findings.

Results

According to network pharmacology prediction, NFE2L2, HMOX1 and PTGS2 may be the key genes for

SUBMITTER: Sun X 

PROVIDER: S-EPMC11654441 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature