Exploring the interplay between yeast cell membrane lipid adaptation and physiological response to acetic acid stress.

ABSTRACT: Acetic acid is a byproduct of lignocellulose pretreatment and a potent inhibitor of yeast-based fermentation processes. A thicker yeast plasma membrane (PM) is expected to retard the passive diffusion of undissociated acetic acid into the cell. Molecular dynamic simulations suggest that membrane thickness can be increased by elongating glycerophospholipids (GPL) fatty acyl chains. Previously, we successfully engineered Saccharomyces cerevisiae to increase GPL fatty acyl chain length but failed to lower acetic acid net uptake. Here, we tested whether altering the relative abundance of diacylglycerol (DAG) might affect PM permeability to acetic acid in cells with longer GPL acyl chains (DAG^EN). To this end, we expressed diacylglycerol kinase α (DGKα) in DAG

SUBMITTER: Wu F 

PROVIDER: S-EPMC11654797 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature