Integrated analysis of BSA-seq and RNA-seq identified the candidate genes for seed weight in Brassica juncea.

ABSTRACT:

Introduction

Brassica juncea is a major oilseed crop of Brassica. The seed weight is one of yield components in oilseed Brassica crops. Research on the genetic mechanism of seed weight is not only directly related to the yield and economic value of Brassica juncea but also can provide a theory foundation for studying other Brassica crops.

Methods

To map the genes for seed weight, the parental and F₂ extreme bulks derived were constructed from the cross between the heavy-seeded accession 7981 and the light-seeded one Sichuan yellow (SY) of B. juncea, and used in bulk segregant sequencing (BSA-seq). Meanwhile, RNA-sequencing (RNA-seq) was performed for both parents at six seed development stages.

Results

Our results showed that a t

SUBMITTER: Yang B 

PROVIDER: S-EPMC11654836 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature