Constructing a doxycycline-inducible system for an epithelial-to-mesenchymal transition model in MCF10A cells.

ABSTRACT: Epithelial to mesenchymal transition (EMT) has been shown to play an essential role in the early stages of cancer cell invasion and metastasis. Inducible EMT models can initiate EMT in a controlled manner, thereby providing the opportunity to determine whether a cancer-associated gene influences cancer metastasis by triggering EMT. Moreover, different inducible EMT models enable the investigation of specific mechanisms of EMT modulation by various genes, facilitating a more precise understanding of how these genes influence cancer metastasis through the induction of EMT. Unfortunately, current inducible EMT models still present unmet needs. Therefore, we aimed to establish an inducible EMT model in MCF10A cells, a spontaneously immortalized human fibrocystic mammary cell line, by manipulat

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11655024 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature