UBR1 Promotes Sex-Dependent ACE2 Ubiquitination in Hypertension.

ABSTRACT:

Background

Ang-II (angiotensin II) impairs the function of the antihypertensive enzyme ACE2 (angiotensin-converting enzyme 2) by promoting its internalization, ubiquitination, and degradation, thus contributing to hypertension. However, few ACE2 ubiquitination partners have been identified, and their role in hypertension remains unknown.

Methods

Proteomics and bioinformatic analyses were used to identify ACE2 ubiquitination partners in the brain, heart, and kidney of hypertensive C57BL6/J mice of both sexes. The interaction between UBR1 (ubiquitin protein ligase E3 component N-recognin) and ACE2 was validated in cells. Central and peripheral UBR1 knockdown was then performed in male mice to investigate its role in the maintenance of hypertension.

Results

Proteomics an

SUBMITTER: Elgazzaz M 

PROVIDER: S-EPMC11655255 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature