First metagenomic sequencing for the analysis of microbial community populations of adults and pupae of <i>Melophagus ovinus</i> in Xinjiang, China.

ABSTRACT:

Introduction

Melophagus ovinus, a parasite on the body surface of sheep, directly attacks the host through biting and sucking blood and may also transmit pathogens in the process. There are currently only a few studies on the microbial composition of M. ovinus, while there are no such studies on pupae.

Methods

In this study, samples AT-1 to AT-4 each contained four M. ovinus individuals, while sample AT-5 comprised four M. ovinus pupae, all used for metagenomic sequencing and analysis. Melophagus ovinus adults and pupae were collected from four regions in Xinjiang, China. DNA was extracted from the samples, amplified, and sequenced using the Illumina Novaseq 6000 System; finally, the sequencing data were analyzed using molecular biology sof

SUBMITTER: Huang K 

PROVIDER: S-EPMC11655492 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature