Proteomic profiling of neonatal herpes simplex virus infection on dried blood spots.

ABSTRACT:

Background

Neonatal herpes simplex virus (HSV) infection is life-threatening, with a mortality of up to 70-80% when disseminated, often due to vague symptoms and delayed treatment. Neonatal screening using dried blood spot (DBS) samples is among the most impactful preventative health measures ever implemented, but screening for HSV has not been investigated.

Methods

We investigated high throughput multiplexed proteomics on DBS samples collected on days 2-3 of life from a nationwide cohort of neonates with HSV infection (n = 53) and matched controls. We measured 2941 proteins using the Olink Explore 3072 panels and proximity extension assays, followed by differential protein expression by Analysis of Variance with post-hoc correction and functional annotation.

Results

SUBMITTER: Dungu KHS 

PROVIDER: S-EPMC11655519 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature