Exploration of In Situ Extraction for Enhanced Triterpenoid Production by Saccharomyces cerevisiae.

ABSTRACT: Plant-derived triterpenoids are in high demand due to their valuable applications in cosmetic, nutraceutical, and pharmaceutical industries. To meet this demand, microbial production of triterpenoids is being developed for large-scale production. However, a prominent limitation of microbial synthesis is the intracellular accumulation, requiring cell disruption during downstream processing. Destroying the whole-cell catalyst drives up production costs and limits productivity and product yield per cell. Here, in situ product extraction of triterpenoids into a second organic phase was researched to address this limitation. An organic solvent screening identified water-immiscible isopropyl myristate as a suitable in situ extractant, enabling extraction of up to 90% of total triterpenoids from

SUBMITTER: Dianat M 

PROVIDER: S-EPMC11655670 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature