ENPP1/CD203a-targeting heavy-chain antibody reveals cell-specific expression on human immune cells.

ABSTRACT: ENPP1/CD203a is a membrane-bound ectonucleotidase capable of hydrolyzing ATP, cGAMP and other substrates. Its enzymatic activity plays an important role in the balance of extracellular adenine nucleotides and the modulation of purinergic signaling, in soft tissue calcification, and in the regulation of the cGAS/STING pathway. However, a detailed analysis of ENPP1 surface expression on human immune cells has not been performed. Here, we selected VHH domains from human ENPP1-immunized alpacas to generate heavy-chain antibodies targeting ENPP1, and analyzed cell surface expression on all circulating immune cell subsets using flow cytometry. We find high expression of ENPP1 in CD141^high conventional dendritic cells (cDC1), while ENPP1 was not detectable on other dendritic cells and

SUBMITTER: Lorenz H 

PROVIDER: S-EPMC11655721 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature