Unveiling hsa_circ_0007439: a novel suppressor of nasopharyngeal carcinoma via miR-556-5p/PTEN Axis.

ABSTRACT:

Objective

There has been no explanation for the exact mechanism of circRNAs in nasopharyngeal carcinoma (NPC). Circ_0007439 was investigated in this study to determine its role and mechanism in NPC.

Methods

To identify circ_0007439, microRNA (miR)-556-5p, and PTEN mRNA in NPC tissues and cells, RT-qPCR was employed. PTEN protein was quantified using western blot analysis. The impact of circ_0007439 on cellular activities was assessed using CCK-8, colony formation, Transwell, and flow cytometry, in that order. The interaction of miR-556-5p with circ_0007439 or PTEN mRNA was confirmed through a dual luciferase reporter gene assay and RIP assay.

Results

circ_0007439 and PTEN levels were low in NPC tissues and cells, while miR-556-5p was highly expressed. NPC cells were i

SUBMITTER: Li M 

PROVIDER: S-EPMC11655796 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature