Non-affinity platform for processing knob-into-hole bispecific antibody.

ABSTRACT: Bispecific antibodies hold significant potential as next-generation biotherapeutics owing to their ability to simultaneously bind to two targets. However, the development of bispecific antibodies as biotherapeutics has been hindered by the high levels of byproducts produced, including both high molecular weight and low molecular weight variants. In addition, the inevitable expression of homodimers in host cells presents further obstacles to the commercial development of bispecific antibodies as therapeutics. These byproducts, which share similar physicochemical properties with the target, pose several challenges for downstream purification processes. In this study, we present a non-protein A purification platform that employ a one-step polishing chromatography to purify bispecific antibodi

SUBMITTER: Wang X 

PROVIDER: S-EPMC11655889 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature