In vitro model of retinoblastoma derived tumor and stromal cells for tumor microenvironment (TME) studies.

ABSTRACT: Retinoblastoma (RB) is an intraocular tumor arising from retinal cone progenitor cells affecting young children. In the last couple of years, RB treatment evolved towards eye preserving therapies. Therefore, investigating intratumoral differences and the RB tumor microenvironment (TME), regulating tumorigenesis and metastasis, is crucial. How RB cells and their TME are involved in tumor development needs to be elucidated using in vitro models including RB derived stromal cells. In the study presented, we established primary RB derived tumor and stromal cell cultures and compared them by RNAseq analysis to identify their gene expression signatures. RB tumor cells cultivated in serum containing medium were more differentiated compared to RB tumor cells grown in serum-free medium displaying a

SUBMITTER: Alefeld E 

PROVIDER: S-EPMC11655973 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature