Transcorneal electrical stimulation restores DNA methylation changes in retinal degeneration.

ABSTRACT:

Background

Retinal degeneration is a major cause of irreversible blindness. Stimulation with controlled low-level electrical fields, such as transcorneal electrical stimulation (TES), has recently been postulated as a therapeutic strategy. With promising results, there is a need for detailed molecular characterization of the therapeutic effects of TES.

Methods

Controlled, non-invasive TES was delivered using a custom contact lens electrode to the retinas of Royal College of Surgeons (RCS) rats, a model of retinal degeneration. DNA methylation in the retina, brain and cell-free DNA in plasma was assessed by reduced representation bisulfite sequencing (RRBS) and gene expression by RNA sequencing.

Results

TES induced DNA methylation and gene expression changes implicated

SUBMITTER: Tew BY 

PROVIDER: S-EPMC11656077 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature