<i>miR-185-5p</i> regulates the proliferation and differentiation of neural stem/progenitor cells.

ABSTRACT:

Background

MicroRNAs (miRNAs) have emerged as an essential regulator of the cell fate commitment of neural stem/progenitor cells (NPCs), although the impacts of certain miRNAs on NPCs remain vague. The aim of this study is to investigate the regulatory effects of miR-185-5p on the cell fate commitment of NPCs.

Methods

We investigated the impact of miR-185-5p on the proliferation and differentiation capacities of primary NPCs by manipulating the expression of miR-185-5p using specific mimics and inhibitors. The effects of miR-185-5p on NPCs was confirmed in vivo through stereotactic injection of miR-185-5p antagonists to the brains of mice at postnatal day 1 (P1).

Results

The expression levels of miR-185-5p kept increasi

SUBMITTER: Feng X 

PROVIDER: S-EPMC11656079 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature