Rolling Circle Amplification Integrating with Exonuclease-III-Assisted Color Reaction for Sensitive Telomerase Activity Analysis.

ABSTRACT: Telomerase activation can lead to the escape from cell senescence and immortalization, playing a crucial role in the growth and proliferation of cancer cells. Therefore, the detection of telomerase activity is essential for cancer diagnosis and treatment. Herein, we develop a novel ultrasensitive and visually detectable platform. By incorporation of exonuclease-III (Exo-III), this platform achieves dual signal amplification of rolling circle amplification products. Additionally, the colorimetric analysis of 3,3',5,5'-tetramethylbiphenyl (TMB) chromogenic reaction system provides this approach with unique advantages such as simplicity, speediness, and sensitivity. The detection platform exhibits high sensitivity and specificity in actual sample testing, which aligns closely with results obt

SUBMITTER: Liu X 

PROVIDER: S-EPMC11656203 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature