Novel Pinhole Emitter Chip for Micro Supercritical Fluid Chromatography-Mass Spectrometry with Integrated Dilution-Free Fluidic Back-Pressure Regulation.

ABSTRACT: We present a novel chip-based device featuring a pinhole emitter for mass spectrometry (MS) coupling with integrated fluidic back-pressure regulation for supercritical mobile phases. This design enables facile coupling of packed capillary columns used for supercritical fluid chromatography (SFC) with atmospheric pressure ionization mass spectrometry. The monolithic microfluidic chips were fabricated using selective laser-induced etching, seamlessly integrating multiple functions, including comb-shaped particle retention structures for column packing and ports for zero-clearance connection with standard fused silica capillaries. The integrated restrictive pinhole MS emitter generated by dielectric breakdown is a key innovation of the micro SFC-MS platform. It enables a controlled decompress

SUBMITTER: Schwieger J 

PROVIDER: S-EPMC11656411 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature