Comprehensive Analysis of CRISPR-Cas Systems and Their Influence on Antibiotic Resistance in <i>Salmonella enterica</i> Strains.

ABSTRACT: Salmonella enterica is a gram-negative bacterium that demonstrates a remarkable ability to acquire antibiotic resistance genes (ARGs). The role of the CRISPR-Cas system in influencing antibiotic resistance in S. enterica is still under investigation. This study explores the distribution and impact of CRISPR-Cas systems on antibiotic resistance by analyzing 316 S. enterica genomes. We conducted sequence alignments, phylogenetic analyses, and conservation studies on Cas genes, direct repeats (DRs), and leader sequences. Promoter predictions and RNA secondary structure analyses were also performed. ARGs were identified, and their correlation with Cas gene clusters was evaluated. Our findings revealed that 82.33% of strains possess complete CRISPR-Cas systems, while 17.66%

SUBMITTER: Fallah T 

PROVIDER: S-EPMC11656426 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature