Aging-related alterations in mechanistic target of rapamycin signaling promote platelet hyperreactivity and thrombosis.

ABSTRACT:

Background

Aging is an independent risk factor for the development of cardiovascular, thrombotic, and other chronic diseases. However, mechanisms of platelet hyperactivation in aging remain poorly understood.

Objectives

Here, we examine whether and how aging alters intracellular signaling in platelets to support platelet hyperactivity and thrombosis.

Methods

Quantitative mass spectrometry with tandem mass tag labeling systematically measured protein phosphorylation in platelets from healthy aged (>65 years) and young human (<45 years) subjects. The role of platelet mechanistic target of rapamycin (mTOR) in aging-induced platelet hyperreactivity was assessed using pharmacologic mTOR inhibition and a platelet-specific mTOR-deficient mouse model (mTOR^plt-/-).<

SUBMITTER: Portier I 

PROVIDER: S-EPMC11656450 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature