Oncogene activated human breast luminal progenitors contribute basally located myoepithelial cells.

ABSTRACT:

Background

Basal-like breast cancer originates in luminal progenitors, frequently with an altered PI3K pathway, and focally in close association with genetically altered myoepithelial cells at the site of tumor initiation. The exact trajectory behind this bi-lineage phenomenon remains poorly understood.

Methods and results

Here we used a breast cancer relevant transduction protocol including hTERT, shp16, shp53, and PIK3CA^H1047R to immortalize FACS isolated luminal cells, and we identified a candidate multipotent progenitor. Specifically, we identified a keratin 23 (K23)⁺/ALDH1A3⁺/CALML5^- ductal-like progenitor with the potential to differentiate into CALML5⁺ lobular-like cells. We found that the apparent luminal phenoty

SUBMITTER: Kohler KT 

PROVIDER: S-EPMC11656586 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature