Dust mite antigens endow dendritic cells with the capacity to induce a Th2 response by regulating their methylation profiles.

ABSTRACT:

Background

It is well-known that Dendritic cells (DCs) are essential in the development of airway Th2 polarization and airway allergy (AA). The underlying mechanism is still not fully understood. The objective of this study is to examine the role of methyltransferase-like protein-5 (Mettl5), a methyltransferase involved in N6-methyladenosine (m6A) methylation, in altering DC's properties to facilitate the development of Th2 polarization and AA.

Methods

Dust mite extracts (DME) were used as a specific antigen to establish an AA mouse model. The epigenetic status of DCs was examined using a Chromatin immunoprecipitation (ChIP) assay. A mouse strain carrying the Mettl5-deficient DCs was used to observe the role of Mettl5 in determining the phenotypes of DCs.

Results

The

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11656822 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature