Inactivation of the conserved protease LonA increases production of xylanase and amylase in Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis is widely used for industrial enzyme production due to its capacity to efficiently secrete proteins. However, secretion efficiency of enzymes varies widely, and optimizing secretion is crucial to make production commercially viable. Previously, we have shown that overexpression of the xylanase XynA lowers expression of Clp protein chaperones, and that inactivation of CtsR, which regulates and represses clp transcription, increases the production of XynA. In the current study, we examined whether the same is the case for overexpression of the α-amylase AmyM from Geobacillus stearothermophilus by B. subtilis, and why XynA shows a different timing of secretion compared to AmyM.

Results

Transcriptome analyses revealed that B. subtilis cells overexpre

SUBMITTER: Wang B 

PROVIDER: S-EPMC11656851 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature