Transcriptome-aligned metabolic profiling by SERSome reflects biological changes following mesenchymal stem cells expansion.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) are widely applied in the treatment of various clinical diseases and in the field of medical aesthetics. However, MSCs exhibit greater heterogeneity limited stability, and more complex molecular and mechanistic characteristics compared to conventional drugs, making rapid and precise monitoring more challenging.

Methods

Surface-enhanced Raman spectroscopy (SERS) is an ultrasensitive, tractable and low-cost fingerprinting technique capable of identifying a wide range of molecules related to biological processes. Here, we employed SERS for reproducible quantification of ultralow concentrations of molecules and utilized spectral sets, termed SERSomes, for robust and comprehensive intracellular multi-metabolite profiling.

Results

We

SUBMITTER: Bi X 

PROVIDER: S-EPMC11656856 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature