Site-Selective Biofunctionalization of 3D Microstructures Via Direct Ink Writing.

ABSTRACT: Two-photon lithography has revolutionized multi-photon 3D laser printing, enabling precise fabrication of micro- and nanoscale structures. Despite many advancements, challenges still persist, particularly in biofunctionalization of 3D microstructures. This study introduces a novel approach combining two-photon lithography with scanning probe lithography for post-functionalization of 3D microstructures overcoming limitations in achieving spatially controlled biomolecule distribution. The method utilizes a diverse range of biomolecule inks, including phospholipids, and two different proteins, introducing high spatial resolution and distinct functionalization on separate areas of the same microstructure. The surfaces of 3D microstructures are treated using bovine serum albumin and/or 3-(Glyci

SUBMITTER: Mathew G 

PROVIDER: S-EPMC11657036 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature