Elucidating siRNA Cellular Delivery Mechanism Mediated by Quaternized Starch Nanoparticles.

ABSTRACT: Starch-based nanoparticles are highly utilized in the realm of drug delivery taking advantage of their biocompatibility and biodegradability. Studies have utilized Quaternized starch (Q-starch) for small interfering RNA (siRNA) delivery, in which quaternary amines enable interaction with negatively charged siRNA, resulting in self-assembly complexation. Although reports present numerous applications, the demonstrated efficacy is nonetheless limited due to undiscovered cellular mechanistic delivery. In this study, a deep dive into Q-starch/siRNA complexes' cellular mechanism and kinetics at the cellular level is revealed using single-particle tracking and cell population level using imaging flow cytometry. Uptake studies depict the efficient cellular internalization via endocytosis while a

SUBMITTER: Amar-Lewis E 

PROVIDER: S-EPMC11657042 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature