Matrix-free human lung organoids derived from induced pluripotent stem cells to model lung injury.

ABSTRACT:

Background

Organoids, as near-physiological 3D culture systems, offer new opportunities to study the pathogenesis of various organs in mimicking the cellular complexity and functionality of human organs.

Method

Here we used a quite simple and very practicable method to successfully generate induced pluripotent stem cell (iPSC)-derived human lung organoids (LuOrg) in a matrix-free manner as an alternative to the widely used preclinical mouse models in order to investigate normal lung damage in detail and as close as possible to the patient. We performed detailed morphological and molecular analyses, including bulk and single cell RNA sequencing, of generated lung organoids and evaluated the quality and robustness of our model as a potential in vitro platform for lung diseases

SUBMITTER: Budeus B 

PROVIDER: S-EPMC11657174 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature