Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell.

ABSTRACT:

Background

Diabetes mellitus (DM) can cause severe complications, including diabetic foot ulcers (DFU). There is a significant gap in understanding the single-cell ecological atlas of DM and DFU tissues.

Methods

Single-cell RNA sequencing data were used to create a detailed single-cell ecological landscape of DM and DFU. Enrichment analysis identified pathways involved in cellular subpopulations, and pseudo-time analysis inferred cell development processes. A gene regulatory network explored the role of transcription factors in DFU progression, and a potential herbal drug-target gene interaction network was constructed.

Results

In the DFU group, immune cells were activated, with notable changes in several subpopulations. ATP5E was significantly overexpressed in Naive

SUBMITTER: Vu L 

PROVIDER: S-EPMC11657181 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature