Prion protein regulates invasiveness in glioblastoma stem cells.

ABSTRACT:

Background

Glioblastoma (GBM) is an aggressive brain tumor driven by glioblastoma stem cells (GSCs), which represent an appealing target for therapeutic interventions. The cellular prion protein (PrP^C), a scaffold protein involved in diverse cellular processes, interacts with various membrane and extracellular matrix molecules, influencing tumor biology. Herein, we investigate the impact of PrP^C expression on GBM.

Methods

To address this goal, we employed CRISPR-Cas9 technology to generate PrP^C knockout (KO) glioblastoma cell lines, enabling detailed loss-of-function studies. Bulk RNA sequencing followed by differentially expressed gene and pathway enrichment analyses between U87 or U251 PrP^C-wild-type (WT) cells and PrP^C

SUBMITTER: Prado MB 

PROVIDER: S-EPMC11657363 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature