In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots.

ABSTRACT:

Background

Nitrate (NO₃^-) is one of the two major forms of inorganic nitrogen absorbed by plant roots, and the tissue nitrate concentration in roots is considered important for optimizing developmental programs. Technologies to quantify the expression levels of nitrate transporters and assimilating enzymes at the cellular level have improved drastically in the past decade. However, a technological gap remains for detecting nitrate at a high spatial resolution. Using extraction-based methods, it is challenging to reliably estimate nitrate concentration from a small volume of cells (i.e., with high spatial resolution), since targeting a small or specific group of cells is physically difficult. Alternatively, nitrate detection with microelectrodes offers subcellu

SUBMITTER: Fernandez Gonzalez A 

PROVIDER: S-EPMC11657419 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature