Comprehensive analysis of lysine lactylation and its potential biological significance in clear cell renal cell carcinoma.

ABSTRACT:

Background

Clear cell renal cell carcinoma (ccRCC) is a common histological subtype of malignant renal neoplasm. Protein lysine lactylation (Kla) plays a crucial role in tumor metabolic reprogramming. However, little is known regarding the distribution and potential biological functions of Kla in ccRCC. This study aimed to systematically investigate the role of Kla in ccRCC.

Methods

A total of 12 ccRCC samples were collected from 6 patients. Western blotting was performed to determine the trend of Kla-modified proteins in ccRCC. Liquid chromatography-tandem mass spectrometry was used to quantitatively analyze Kla in ccRCC. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) network analyses were conducted to clarify the b

SUBMITTER: Wan B 

PROVIDER: S-EPMC11657551 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature