High-efficiency production of plant-derived pigment dopaxanthin in Escherichia coli by combination engineering.

ABSTRACT:

Background

Dopaxanthin is a natural pigment betaxanthins family member with the highest antioxidant and free radical scavenging activities. However, its relatively low content in plants limited the wide range of applications. Cost-efficient microbial production, therefore, showed an attractive alternative.

Results

Here, an Escherichia coli strain equipped with the de novo biosynthetic pathway for hyperproducing dopaxanthin was constructed by combining metabolic engineering and protein engineering. Firstly, a high-performance rate-limiting levodopa 4,5-dioxygenase (DODA) was mined and characterized based on sequence similarity searching followed by whole-cell catalysis and de novo synthesis strategy. Then, the catalytic efficiency of DODA was increased 34 times with directed

SUBMITTER: Jiang X 

PROVIDER: S-EPMC11657626 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature