HPV11 targeting KDM4A regulates the polarization of macrophage M₁ and promotes the development of nasal inverted papilloma.

ABSTRACT: The development of nasal inverted papilloma (NIP) is closely related to human papillomavirus (HPV) infection. Previous studies indicated that HPV11 shows the highest expression in NIP tissues. However, the mechanisms following its integration into host DNA require further clarification. In this study, high-throughput sequencing was employed to identify the HPV integration site KDM4A in HPV-positive specimens. The HPV11E6/E7 overexpression model was established in human nasal mucosal epithelial cells (HNE-pC), and the KDM4A gene was knocked out using CRISPR/Cas9 technology. Cell proliferation was assessed via CCK-8, colony formation, and EdU assays, while cell migration was evaluated through Transwell and wound healing assays. qRT-PCR and Western blot were used not only to analyze mRNA and

SUBMITTER: Zheng L 

PROVIDER: S-EPMC11657743 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature