Proteoglycan 4 (Lubricin) and regulation of xanthine oxidase in synovial macrophage as a mechanism of controlling synovitis.

ABSTRACT:

Background

Synovial macrophages (SMs) are important effectors of joint health and disease. A novel Cx3CR1 + TREM2 + SM population expressing the tight junction protein claudin-5, was recently discovered in synovial lining. Ablation of these SMs was associated with onset of arthritis. Proteoglycan 4 (PRG4) is a mucinous glycoprotein that fulfills lubricating and homeostatic roles in the joint. The aim of this work is to study the role of PRG4 in modulating synovitis in the context of SM homeostasis and assess the contribution of xanthine oxidase (XO)-hypoxia inducible factor alpha (HIF-1a) axis to this regulation.

Methods

We used Prg4^{FrtloxP/FrtloxP};R26^FlpoER/+, a novel transgenic mouse, where the Prg4^Frt allele normally expresses the PRG4 pro

SUBMITTER: Elsaid KA 

PROVIDER: S-EPMC11657766 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature