Loop-mediated isothermal amplification assays for the detection of antimicrobial resistance elements in Vibrio cholera.

ABSTRACT:

Background

The bacterium Vibrio cholerae causes diarrheal illness and can acquire genetic material leading to multiple drug resistance (MDR). Rapid detection of resistance-conferring mobile genetic elements helps avoid the prescription of ineffective antibiotics for specific strains. Colorimetric loop-mediated isothermal amplification (LAMP) assays provide a rapid and cost-effective means for detection at point-of-care since they do not require specialized equipment, require limited expertise to perform, and can take less than 30 min to perform in resource limited regions. LAMP output is a color change that can be viewed by eye, but it can be difficult to design primer sets, determine target specificity, and interpret subjective color changes.

Methods

We developed an algorit

SUBMITTER: Negron DA 

PROVIDER: S-EPMC11657800 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature