Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.

ABSTRACT:

Significance

Cellular metabolic dynamics can occur within milliseconds, yet there are no optimal tools to spatially and temporally capture these events. Autofluorescence imaging can provide metabolic information on the cellular level due to the intrinsic fluorescence of reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD).

Aim

Our goal is to build and evaluate a widefield microscope optimized for rapid autofluorescence imaging of metabolic changes in cells.

Approach

A widefield, fluorescence microscope was assembled from an inverted microscope base, an light-emitting diode (LED) for excitation, and an image splitter for simultaneous but separate imaging of two bandwidths of emission (451/106 and 560/94 nm) on a single s

SUBMITTER: Theodossiou A 

PROVIDER: S-EPMC11657876 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature