Microglial CD2AP deficiency exerts protection in an Alzheimer's disease model of amyloidosis.

ABSTRACT:

Background

The CD2-associated protein (CD2AP) was initially identified in peripheral immune cells and regulates cytoskeleton and protein trafficking. Single nucleotide polymorphisms (SNPs) in the CD2AP gene have been associated with Alzheimer's disease (AD). However, the functional role of CD2AP, especially its role in microglia during AD onset, remains elusive.

Methods

CD2AP protein levels in cultured primary cells and in 5xFAD mice was studied. Microglial CD2AP-deficient mice were crossed with 5xFAD mice and the offspring were subjected to neuropathological assessment, behavioral tests, electrophysiology, RNA-seq, Golgi staining, and biochemistry analysis. Primary microglia were also isolated for assessing their uptake and morphology changes.

Results

We find that CD

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11658232 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature