Oscillatory dynamics of Rac1 activity in Dictyostelium discoideum amoebae.

ABSTRACT: Small GTPases of the Rho family play a central role in the regulation of cell motility by controlling the remodeling of the actin cytoskeleton. In the amoeboid cells of Dictyostelium discoideum, the active form of the Rho GTPase Rac1 regulates actin polymerases at the leading edge and actin filament bundling proteins at the posterior cortex of polarized cells. We monitored the spatiotemporal dynamics of Rac1 and its effector DGAP1 in vegetative amoebae using specific fluorescent probes. We observed that plasma membrane domains enriched in active Rac1 not only exhibited stable polarization, but also showed rotations and oscillations, whereas DGAP1 was depleted from these regions. To simulate the observed dynamics of the two proteins, we developed a mass-conserving reaction-diffusion model b

SUBMITTER: Sostar M 

PROVIDER: S-EPMC11658709 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature