Statistical analysis supports pervasive RNA subcellular localization and alternative 3' UTR regulation.

ABSTRACT: Targeted low-throughput studies have previously identified subcellular RNA localization as necessary for cellular functions including polarization, and translocation. Furthermore, these studies link localization to RNA isoform expression, especially 3' Untranslated Region (UTR) regulation. The recent introduction of genome-wide spatial transcriptomics techniques enables the potential to test if subcellular localization is regulated in situ pervasively. In order to do this, robust statistical measures of subcellular localization and alternative poly-adenylation (APA) at single-cell resolution are needed. Developing a new statistical framework called SPRAWL, we detect extensive cell-type specific subcellular RNA localization regulation in the mouse brain and to a lesser extent mouse liver. W

SUBMITTER: Bierman R 

PROVIDER: S-EPMC11658768 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature