Analytical and Biological Variability of a Commercial Modified Aptamer Assay in Plasma Samples of Patients with Chronic Kidney Disease.

ABSTRACT:

Background

We carried out a study of the aptamer proteomic assay, SomaScan V4, to evaluate the analytical and biological variability of the assay in plasma samples of patients with moderate to severe chronic kidney disease (CKD).

Methods

Plasma samples were selected from 2 sources: (a) 24 participants from the Chronic Renal Insufficiency Cohort (CRIC) and (b) 49 patients from the Brigham and Women's Hospital-Kidney/Renal Clinic. We calculated intra-assay variability from both sources and examined short-term biological variability in samples from the Brigham clinic. We also measured correlations of aptamer measurements with traditional biomarker assays.

Results

A total of 4656 unique proteins (4849 total aptamer measures) were analyzed in all samples. Median (interquar

SUBMITTER: Dubin RF 

PROVIDER: S-EPMC11658805 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature