Discovery of quality markers of <i>Meconopsis quintuplinervia</i> based on an absorption-based metabolite approach and rapid quantification using polarity-switching UPLC-QQQ-MS/MS.

ABSTRACT:

Background

Meconopsis quintuplinervia Regel (MQ) is a traditional Chinese medicine (TCM) used for clearing liver and lung heat in Tibetan medicine for over a thousand years. However, the lack of quality markers that correlate with pharmacological actions and absorption in vivo limits the safety and efficacy in its clinical application and on the drug market. Furthermore, a universal and rapid method for simultaneously determining quality markers in the MQ crude drug is still lacking.

Purpose

An absorption-based metabolite approach was used to discover and identify the quality markers of MQ. An efficient method based on polarity-switching ultra-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS) was then established to determin

SUBMITTER: Tian Y 

PROVIDER: S-EPMC11658988 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature