CsRAB, a R2R3-MYB transcription factor from purple tea (Camellia sinensis), positively regulates anthocyanin biosynthesis.

ABSTRACT: In tea (Camellia sinensis), anthocyanins are important secondary metabolites that are linked to leaf color. Anthocyanin biosynthesis is a complex biological process, in which multiple genes including structural and regulatory genes are involved. Here, we describe the cloning and characterizing of a new R2R3-MYB transcription factor gene, CsRAB, isolated from purple tea variety 'Hongfei'. Consistent with its predicted role as a transcription factor, the CsRAB protein localized to nuclei when expressed in onion (Allium cepa) epidermal cell. A dual-luciferase reporter assay demonstrated that CsRAB acts as a transcriptional activator in vivo. CsRAB overexpression in Arabidopsis seedlings led to higher expression levels of anthocyanin biosynthesi

SUBMITTER: Wu H 

PROVIDER: S-EPMC11658990 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature