Analysis of bb0556 Expression and Its Role During Borrelia burgdorferi Mammalian Infection.

ABSTRACT: In Borrelia burgdorferi, BB0556 was annotated as a conserved hypothetical protein. We herein investigated gene expression and the importance of this protein during infection. Our data support that bb0556 forms an operon with five other genes. A transcriptional start site and the associated σ⁷⁰-type promoter were identified in the sequences upstream of bb0554, and luciferase reporter assays indicated that this promoter is functional in B. burgdorferi. Furthermore, the sequences upstream of bb0556 contain an internal promoter to drive gene expression. bb0556 expression was affected by various environmental factors such as changes in temperature, pH, and cell density when B. burgdorferi was grown in vitro. Surprisingly, significant differences were observed for bb0556 expression be

SUBMITTER: George S 

PROVIDER: S-EPMC11659052 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature