Transcriptional regulation in the absence of inositol trisphosphate receptor calcium signaling.

ABSTRACT: The activation of IP₃ receptor (IP₃R) Ca²⁺ channels generates agonist-mediated Ca²⁺ signals that are critical for the regulation of a wide range of biological processes. It is therefore surprising that CRISPR induced loss of all three IP₃R isoforms (TKO) in HEK293 and HeLa cell lines yields cells that can survive, grow and divide, albeit more slowly than wild-type cells. In an effort to understand the adaptive mechanisms involved, we have examined the activity of key Ca²⁺ dependent transcription factors (NFAT, CREB and AP-1) and signaling pathways using luciferase-reporter assays, phosphoprotein immunoblots and whole genome transcriptomic studies. In addition, the diacylglycerol arm of the signaling pathway was investigated

SUBMITTER: Young M 

PROVIDER: S-EPMC11659226 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature