Two detoxification enzyme genes, <i>CYP6DA2</i> and <i>CarFE4</i>, mediate the susceptibility to afidopyropen in <i>Semiaphis heraclei</i>.

ABSTRACT:

Introduction

Semiaphis heraclei is an important economic pest affecting Caprifoliaceae and Apiaceae plants, and chemical control is still the main effective control method in the field. Afidopyropen is a new type of pyridine cyclopropyl insecticide, which can effectively control piercing-sucking mouthparts pests and is suitable for pest resistance management. However, the detoxification mechanism of S. heraclei to afidopyropen is still poorly cleared.

Methods

The insecticidal activity of afidopyropen against S. heraclei and the enzyme activity assay and synergism bioassay were evaluated. The detoxification enzyme genes were obtained by transcriptome and validated by quantitative real-time PCR (RT-qPCR). Furthermore, RNA interference was used to st

SUBMITTER: Fu X 

PROVIDER: S-EPMC11659293 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature